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tcf wild-type (topflash) or mutated control (fopflash) luciferase reporter plasmids  (Upstate Biotechnology Inc)

 
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    Upstate Biotechnology Inc tcf wild-type (topflash) or mutated control (fopflash) luciferase reporter plasmids
    Tcf Wild Type (Topflash) Or Mutated Control (Fopflash) Luciferase Reporter Plasmids, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutated+control+reporter+fopflash/tcf+reporter+plasmid+topflash/pmc05964149-184-0-13
    Average 90 stars, based on 1 article reviews
    tcf wild-type (topflash) or mutated control (fopflash) luciferase reporter plasmids - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Dominant Negative Mutation:

    Article Title: E-cadherin inhibits tumor cell growth by suppressing PI3K/Akt signaling via β-catenin-Egr1-mediated PTEN expression.
    Article Snippet: Dominant-negative Akt, the TCF-binding site reporter plasmid (TOPFLASH) and a mutated control reporter (FOPFLASH) were purchased from Upstate Biotechnology (Lake Placid, NY, USA).

    Article Title: Functional localization and competition between the androgen receptor and T-cell factor for nuclear beta-catenin: a means for inhibition of the Tcf signaling axis.
    Article Snippet: The Tcf4 luciferase reporter (TOPFLASH) and a mutated control reporter (FOPFLASH) were purchased from Upstate Biotechnology.

    Plasmid Preparation:

    Article Title: E-cadherin inhibits tumor cell growth by suppressing PI3K/Akt signaling via β-catenin-Egr1-mediated PTEN expression.
    Article Snippet: Dominant-negative Akt, the TCF-binding site reporter plasmid (TOPFLASH) and a mutated control reporter (FOPFLASH) were purchased from Upstate Biotechnology (Lake Placid, NY, USA).

    Article Title: Functional localization and competition between the androgen receptor and T-cell factor for nuclear beta-catenin: a means for inhibition of the Tcf signaling axis.
    Article Snippet: The Tcf4 luciferase reporter (TOPFLASH) and a mutated control reporter (FOPFLASH) were purchased from Upstate Biotechnology.

    Control:

    Article Title: E-cadherin inhibits tumor cell growth by suppressing PI3K/Akt signaling via β-catenin-Egr1-mediated PTEN expression.
    Article Snippet: Dominant-negative Akt, the TCF-binding site reporter plasmid (TOPFLASH) and a mutated control reporter (FOPFLASH) were purchased from Upstate Biotechnology (Lake Placid, NY, USA).

    Article Title: Functional localization and competition between the androgen receptor and T-cell factor for nuclear beta-catenin: a means for inhibition of the Tcf signaling axis.
    Article Snippet: The Tcf4 luciferase reporter (TOPFLASH) and a mutated control reporter (FOPFLASH) were purchased from Upstate Biotechnology.

    Luciferase:

    Article Title: E-cadherin inhibits tumor cell growth by suppressing PI3K/Akt signaling via β-catenin-Egr1-mediated PTEN expression.
    Article Snippet: Dominant-negative Akt, the TCF-binding site reporter plasmid (TOPFLASH) and a mutated control reporter (FOPFLASH) were purchased from Upstate Biotechnology (Lake Placid, NY, USA).

    Article Title: Functional localization and competition between the androgen receptor and T-cell factor for nuclear beta-catenin: a means for inhibition of the Tcf signaling axis.
    Article Snippet: The Tcf4 luciferase reporter (TOPFLASH) and a mutated control reporter (FOPFLASH) were purchased from Upstate Biotechnology.



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    tcf wild-type (topflash) mutated control (fopflash) luciferase reporter plasmids  (Upstate Biotechnology Inc)
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    Upstate Biotechnology Inc tcf wild-type (topflash) mutated control (fopflash) luciferase reporter plasmids
    Effect of altered Fat1 expression on β-catenin transcriptional activity in VSMCs. (A) <t>Topflash</t> <t>(TCF-luciferase</t> reporter) activation. Topflash or Fopflash control was transfected into A7r5 cells along with expression constructs for β-catenin, IL2R-Fat1 IC , N-cadherin, and/or IL2R-E-cadherin IC . The maximal reporter activity was set to 100. *, P < 0.05, **, P < 0.01, vs. activity with β-catenin alone. (B) β-Catenin localization in MASMCs transfected with control (Ctl, scrambled) or Fat1-specific (7296) siRNAs and stimulated with LiCl (20 mM) for 12 h. (C) Topflash activity with decreased Fat1 expression. MASMCs transfected with the indicated siRNAs and the Topflash reporter were stimulated with LiCl (20 mM) for 12 h before assay for luciferase activity. *, P < 0.05, vs. activity with Ctl siRNA. (D) Cyclin D1 promoter activation. The cyclin D1 promoter-luciferase construct was transfected into A7r5 cells along with test constructs, as in A. Data show the means ± SEM. *, P < 0.05, **, P < 0.01, vs. activity with β-catenin alone.
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    Image Search Results


    Effect of altered Fat1 expression on β-catenin transcriptional activity in VSMCs. (A) Topflash (TCF-luciferase reporter) activation. Topflash or Fopflash control was transfected into A7r5 cells along with expression constructs for β-catenin, IL2R-Fat1 IC , N-cadherin, and/or IL2R-E-cadherin IC . The maximal reporter activity was set to 100. *, P < 0.05, **, P < 0.01, vs. activity with β-catenin alone. (B) β-Catenin localization in MASMCs transfected with control (Ctl, scrambled) or Fat1-specific (7296) siRNAs and stimulated with LiCl (20 mM) for 12 h. (C) Topflash activity with decreased Fat1 expression. MASMCs transfected with the indicated siRNAs and the Topflash reporter were stimulated with LiCl (20 mM) for 12 h before assay for luciferase activity. *, P < 0.05, vs. activity with Ctl siRNA. (D) Cyclin D1 promoter activation. The cyclin D1 promoter-luciferase construct was transfected into A7r5 cells along with test constructs, as in A. Data show the means ± SEM. *, P < 0.05, **, P < 0.01, vs. activity with β-catenin alone.

    Journal: The Journal of Cell Biology

    Article Title: The Fat1 cadherin integrates vascular smooth muscle cell growth and migration signals

    doi: 10.1083/jcb.200508121

    Figure Lengend Snippet: Effect of altered Fat1 expression on β-catenin transcriptional activity in VSMCs. (A) Topflash (TCF-luciferase reporter) activation. Topflash or Fopflash control was transfected into A7r5 cells along with expression constructs for β-catenin, IL2R-Fat1 IC , N-cadherin, and/or IL2R-E-cadherin IC . The maximal reporter activity was set to 100. *, P < 0.05, **, P < 0.01, vs. activity with β-catenin alone. (B) β-Catenin localization in MASMCs transfected with control (Ctl, scrambled) or Fat1-specific (7296) siRNAs and stimulated with LiCl (20 mM) for 12 h. (C) Topflash activity with decreased Fat1 expression. MASMCs transfected with the indicated siRNAs and the Topflash reporter were stimulated with LiCl (20 mM) for 12 h before assay for luciferase activity. *, P < 0.05, vs. activity with Ctl siRNA. (D) Cyclin D1 promoter activation. The cyclin D1 promoter-luciferase construct was transfected into A7r5 cells along with test constructs, as in A. Data show the means ± SEM. *, P < 0.05, **, P < 0.01, vs. activity with β-catenin alone.

    Article Snippet: A7r5 cells growing in DMEM supplemented with 10% FBS were transfected transiently using Lipofectamine 2000 with β-catenin, IL2R-Fat1 IC , Fat1 4189–4587 , Fat1 4201–4587 , or control expression constructs, along with the TCF wild-type (Topflash) and mutated control (Fopflash) luciferase reporter plasmids (Upstate Biotechnology), or cyclin D1 promoter luciferase reporter (a gift from R. Müller, Philipps-Universität, Marburg, Germany; ).

    Techniques: Expressing, Activity Assay, Luciferase, Activation Assay, Transfection, Construct